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southern biotech 1090 05  (SouthernBiotech)


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    SouthernBiotech southern biotech 1090 05
    Southern Biotech 1090 05, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 126 article reviews
    southern biotech 1090 05 - by Bioz Stars, 2026-08
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    Figure 3. Expansion of T-bet+ CD11c+ B cells in diet-induced obesity depends on iNKT cells (A) Flow cytometry of lymphocytes from WT or Cd1d1/ mice fed the NCD or HFD, presented with the t-SNE algorithm. See also Figure S4. Color in top row cell maps indicates expression of CD19: blue (low) to red (high). Second row cell maps indicate subgating of B cell cluster 1 (blue) and 2 (red). Histograms show expression of T-bet (third row) and CD11c (bottom row) by clusters identified in t-SNE maps: B cell cluster 1 (blue) and 2 (red). (B–D) Representative flow cytometry of splenic (B) and quantification of WAT and splenic (C and D) T-bet+ CD11c+ cell frequency of total B cells. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). (E) Flow cytometric quantification of numbers of NK1.1+ iNKT cells in WAT from WT mice fed an NCD (gray) or HFD (red). (F and G) ELISA measure of serum IgG1 (F), <t>IgG2c</t> (G), and IgM (H) antibodies from WT or Cd1d1/ mice fed the NCD or HFD. There is a correlation between frequency of splenic T-bet+ CD11c+ B cells and IgG1 (F), IgG2c (G), and IgM (H), assessed by flow cytometry and ELISA, respectively. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). Dots represent individual mice. Data pooled from 7 (A–D), 2 (E), or 3 (F and G) experiments with at least 3 mice/group. Mean ± SEM; two-way ANOVA (B and C) or Student’s t test and Student’s paired t test (D–F). Lines indicate linear regression trend with coefficient of determination (r2); *p < 0.05, **p < 0.01, ***p < 0.001.
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    Figure 3. Expansion of T-bet+ CD11c+ B cells in diet-induced obesity depends on iNKT cells (A) Flow cytometry of lymphocytes from WT or Cd1d1/ mice fed the NCD or HFD, presented with the t-SNE algorithm. See also Figure S4. Color in top row cell maps indicates expression of CD19: blue (low) to red (high). Second row cell maps indicate subgating of B cell cluster 1 (blue) and 2 (red). Histograms show expression of T-bet (third row) and CD11c (bottom row) by clusters identified in t-SNE maps: B cell cluster 1 (blue) and 2 (red). (B–D) Representative flow cytometry of splenic (B) and quantification of WAT and splenic (C and D) T-bet+ CD11c+ cell frequency of total B cells. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). (E) Flow cytometric quantification of numbers of NK1.1+ iNKT cells in WAT from WT mice fed an NCD (gray) or HFD (red). (F and G) ELISA measure of serum IgG1 (F), <t>IgG2c</t> (G), and IgM (H) antibodies from WT or Cd1d1/ mice fed the NCD or HFD. There is a correlation between frequency of splenic T-bet+ CD11c+ B cells and IgG1 (F), IgG2c (G), and IgM (H), assessed by flow cytometry and ELISA, respectively. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). Dots represent individual mice. Data pooled from 7 (A–D), 2 (E), or 3 (F and G) experiments with at least 3 mice/group. Mean ± SEM; two-way ANOVA (B and C) or Student’s t test and Student’s paired t test (D–F). Lines indicate linear regression trend with coefficient of determination (r2); *p < 0.05, **p < 0.01, ***p < 0.001.
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    Figure 3. Expansion of T-bet+ CD11c+ B cells in diet-induced obesity depends on iNKT cells (A) Flow cytometry of lymphocytes from WT or Cd1d1/ mice fed the NCD or HFD, presented with the t-SNE algorithm. See also Figure S4. Color in top row cell maps indicates expression of CD19: blue (low) to red (high). Second row cell maps indicate subgating of B cell cluster 1 (blue) and 2 (red). Histograms show expression of T-bet (third row) and CD11c (bottom row) by clusters identified in t-SNE maps: B cell cluster 1 (blue) and 2 (red). (B–D) Representative flow cytometry of splenic (B) and quantification of WAT and splenic (C and D) T-bet+ CD11c+ cell frequency of total B cells. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). (E) Flow cytometric quantification of numbers of NK1.1+ iNKT cells in WAT from WT mice fed an NCD (gray) or HFD (red). (F and G) ELISA measure of serum IgG1 (F), <t>IgG2c</t> (G), and IgM (H) antibodies from WT or Cd1d1/ mice fed the NCD or HFD. There is a correlation between frequency of splenic T-bet+ CD11c+ B cells and IgG1 (F), IgG2c (G), and IgM (H), assessed by flow cytometry and ELISA, respectively. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). Dots represent individual mice. Data pooled from 7 (A–D), 2 (E), or 3 (F and G) experiments with at least 3 mice/group. Mean ± SEM; two-way ANOVA (B and C) or Student’s t test and Student’s paired t test (D–F). Lines indicate linear regression trend with coefficient of determination (r2); *p < 0.05, **p < 0.01, ***p < 0.001.
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    Figure 3. Expansion of T-bet+ CD11c+ B cells in diet-induced obesity depends on iNKT cells (A) Flow cytometry of lymphocytes from WT or Cd1d1/ mice fed the NCD or HFD, presented with the t-SNE algorithm. See also Figure S4. Color in top row cell maps indicates expression of CD19: blue (low) to red (high). Second row cell maps indicate subgating of B cell cluster 1 (blue) and 2 (red). Histograms show expression of T-bet (third row) and CD11c (bottom row) by clusters identified in t-SNE maps: B cell cluster 1 (blue) and 2 (red). (B–D) Representative flow cytometry of splenic (B) and quantification of WAT and splenic (C and D) T-bet+ CD11c+ cell frequency of total B cells. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). (E) Flow cytometric quantification of numbers of NK1.1+ iNKT cells in WAT from WT mice fed an NCD (gray) or HFD (red). (F and G) ELISA measure of serum IgG1 (F), <t>IgG2c</t> (G), and IgM (H) antibodies from WT or Cd1d1/ mice fed the NCD or HFD. There is a correlation between frequency of splenic T-bet+ CD11c+ B cells and IgG1 (F), IgG2c (G), and IgM (H), assessed by flow cytometry and ELISA, respectively. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). Dots represent individual mice. Data pooled from 7 (A–D), 2 (E), or 3 (F and G) experiments with at least 3 mice/group. Mean ± SEM; two-way ANOVA (B and C) or Student’s t test and Student’s paired t test (D–F). Lines indicate linear regression trend with coefficient of determination (r2); *p < 0.05, **p < 0.01, ***p < 0.001.
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    Figure 3. Expansion of T-bet+ CD11c+ B cells in diet-induced obesity depends on iNKT cells (A) Flow cytometry of lymphocytes from WT or Cd1d1/ mice fed the NCD or HFD, presented with the t-SNE algorithm. See also Figure S4. Color in top row cell maps indicates expression of CD19: blue (low) to red (high). Second row cell maps indicate subgating of B cell cluster 1 (blue) and 2 (red). Histograms show expression of T-bet (third row) and CD11c (bottom row) by clusters identified in t-SNE maps: B cell cluster 1 (blue) and 2 (red). (B–D) Representative flow cytometry of splenic (B) and quantification of WAT and splenic (C and D) T-bet+ CD11c+ cell frequency of total B cells. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). (E) Flow cytometric quantification of numbers of NK1.1+ iNKT cells in WAT from WT mice fed an NCD (gray) or HFD (red). (F and G) ELISA measure of serum IgG1 (F), IgG2c (G), and IgM (H) antibodies from WT or Cd1d1/ mice fed the NCD or HFD. There is a correlation between frequency of splenic T-bet+ CD11c+ B cells and IgG1 (F), IgG2c (G), and IgM (H), assessed by flow cytometry and ELISA, respectively. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). Dots represent individual mice. Data pooled from 7 (A–D), 2 (E), or 3 (F and G) experiments with at least 3 mice/group. Mean ± SEM; two-way ANOVA (B and C) or Student’s t test and Student’s paired t test (D–F). Lines indicate linear regression trend with coefficient of determination (r2); *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Cell metabolism

    Article Title: T-bet + B cells accumulate in adipose tissue and exacerbate metabolic disorder during obesity.

    doi: 10.1016/j.cmet.2022.07.002

    Figure Lengend Snippet: Figure 3. Expansion of T-bet+ CD11c+ B cells in diet-induced obesity depends on iNKT cells (A) Flow cytometry of lymphocytes from WT or Cd1d1/ mice fed the NCD or HFD, presented with the t-SNE algorithm. See also Figure S4. Color in top row cell maps indicates expression of CD19: blue (low) to red (high). Second row cell maps indicate subgating of B cell cluster 1 (blue) and 2 (red). Histograms show expression of T-bet (third row) and CD11c (bottom row) by clusters identified in t-SNE maps: B cell cluster 1 (blue) and 2 (red). (B–D) Representative flow cytometry of splenic (B) and quantification of WAT and splenic (C and D) T-bet+ CD11c+ cell frequency of total B cells. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). (E) Flow cytometric quantification of numbers of NK1.1+ iNKT cells in WAT from WT mice fed an NCD (gray) or HFD (red). (F and G) ELISA measure of serum IgG1 (F), IgG2c (G), and IgM (H) antibodies from WT or Cd1d1/ mice fed the NCD or HFD. There is a correlation between frequency of splenic T-bet+ CD11c+ B cells and IgG1 (F), IgG2c (G), and IgM (H), assessed by flow cytometry and ELISA, respectively. WT NCD (white) and HFD (black) or Cd1d1/ NCD (light blue) and HFD (dark blue). Dots represent individual mice. Data pooled from 7 (A–D), 2 (E), or 3 (F and G) experiments with at least 3 mice/group. Mean ± SEM; two-way ANOVA (B and C) or Student’s t test and Student’s paired t test (D–F). Lines indicate linear regression trend with coefficient of determination (r2); *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER CD3 BV510 Biolegend 317332; RRID: AB_2561943 Va24-Ja18 Brilliant Violet 421 Biolegend 342915; RRID: AB_2564004 CD15 FITC Biolegend 301904; RRID: AB_314196 T-bet BV785 Biolegend 644835; RRID: AB_2721566 Tbet PECy7 Biolegend 644824; RRID: AB_2561761 Isotype MOPC-21; IgG1 k BV785 Biolegend 400169; RRID: AB_11219601 CD11c BV650 Biolegend 301637; RRID: AB_2562231 CD69 BV650 Biolegend 310933; RRID: AB_2561783 CD27 APC Clone O323 Tonbo 20-0279-T025; RRID: AB_2621569 CD27 Clone LG.3A10 Biolegend 124211; RRID: AB_1236460 IgD BV421 Biolegend 348225; RRID: AB_2561618 Total capture Invitrogen PA1-85986 IgG Horseradish Peroxidase Southern Biotech 1030-05 IgM Horseradish Peroxidase Southern Biotech 1020-05 IgG1 Horseradish Peroxidase Southern Biotech 1070-05 IgG2b Horseradish Peroxidase Southern Biotech 1090-05 IgG2c Horseradish Peroxidase Southern Biotech 1078-05 IgG3 Horseradish Peroxidase Southern Biotech 1100-05 IgA Horseradish Peroxidase Southern Biotech 1040-05 IgE Horseradish Peroxidase Southern Biotech 1110-05 Total capture Southern Biotech 2010-01 IgG Horseradish Peroxidase Jackson ImmunoResearch N/A IgM Horseradish Peroxidase Southern Biotech 2020-05 IgG1 Horseradish Peroxidase Southern Biotech 9052-05 IgG2 Horseradish Peroxidase Southern Biotech 9060-05 IgG3 Horseradish Peroxidase Southern Biotech 9210-05 IgG4 Horseradish Peroxidase Southern Biotech 9200-05 IgA Horseradish Peroxidase Jackson ImmunoResearch N/A IgE Horseradish Peroxidase Southern Biotech 9160-05 Rabbit Serum Fisher; Sigma ICN1935780; R4505-500 Goat Serum Sigma G6767 Biological samples Human samples San Antonio Plastic Surgery Institute/Christus Santa Rosa Hospital N/A Chemicals, peptides, and recombinant proteins Alpha Galactosyl-Ceramide KRN7000 Enzo Life Sciences; Avanti Polar lipids Cat# BML-SL232; 867000P-1mg Resiquimod Sigma SML0196-10MG CD1d-tetramers NIH Tetramer Core Facility N/A Brefeldin A (GolgiPlug) Biolegend 420601 FBS Cytiva SH30088.03 L-Glutamine Fisher 25-005-CI RPMI 1640 medium Fisher MT10040CV Collagenase Sigma C5138-5G Streptavidin-Horseradish Peroxidase Fisher; BD Pharmingen SNN2004 (nowS911); 554066 Glucose Sigma G8769-100ML Formalin Fisher 22-050-104 OCT Fisher 23-730-571 (Continued on next page) e2 Cell Metabolism 34, 1121–1136.e1–e6, August 2, 2022

    Techniques: Flow Cytometry, Expressing, Cytometry, Enzyme-linked Immunosorbent Assay